Arl2 Associates with Cdk5rap2 to Regulate Cortical Development via Microtubule Organization
Ma, D.; Lin, K.-Y.; Divya, S.; Lin, J.; Gujar, M. R.; Aung, H. Y.; Tan, Y. S.; Gao, Y.; Vincent, A. S.; Chen, T.; Wang, H.
Show abstract
ADP ribosylation factor-like GTPase 2 (Arl2) is crucial for controlling mitochondrial fusion and microtubule assembly in various organisms. Arl2 regulates the asymmetric division of neural stem cells in Drosophila via microtubule growth. However, the function of mammalian Arl2 during cortical development was unknown. Here, we demonstrate that mouse Arl2 plays a new role in corticogenesis via regulating microtubule growth, but not mitochondria functions. Arl2 knockdown leads to impaired proliferation of neural progenitor cells (NPCs) and neuronal migration. Arl2 knockdown in mouse NPCs significantly diminishes centrosomal microtubule growth and delocalization of centrosomal proteins Cdk5rap2 and {gamma}-tubulin. Moreover, Arl2 physically associates with Cdk5rap2 by in silico prediction using AlphaFold Multimer and in vitro binding assays. Remarkably, Cdk5rap2 overexpression significantly rescues the neurogenesis defects caused by Arl2 knockdown. Therefore, Arl2 plays an important role in mouse cortical development through microtubule growth via the centrosomal protein Cdk5rap2.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The non-mitotic role of HMMR in regulating the localization of TPX2 and the dynamics of microtubules in neurons 95%
- Teneurin-2 at the Synapse Construction Site is a Signpost for Cargo Unloading from Motor Proteins 95%
- Embryonic origins of forebrain oligodendrocytes revisited by combinatorial genetic fate mapping 95%
Similar papers in this journal
- Impaired p53-mediated DNA damage response contributes to microcephaly in Nijmegen Breakage Syndrome patient-derived cerebral organoids 94%
- C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stress 94%
- Functional characterization of Neurofilament Light b splicing and misbalance in zebrafish 94%
Similar papers in this journal
- Centrosome-dependent microtubule organization sets the conditions for axon formation 97%
- Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins 95%
- Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.