High-throughput analysis of membrane fluidity unveils a hidden dimension in immune cell states
Andronico, L. A.; Jiang, Y.; Carannante, V.; Iskrak, S.; Sandoz, P. A.; Mikes, J.; Klymchenko, A. S.; Buggert, M.; Osterborg, A.; Onfelt, B.; Brodin, P.; Sezgin, E.
Show abstract
Cell membranes undergo biophysical remodelling as an adaptation to the surroundings and to perform specific biological functions. However, the extent and relevance of such changes in human immune cells remain unknown, largely due to the lack of high throughput and multidimensional methodologies. Here, we describe a cytometry-based method with single-cell resolution which fills this gap by combining biophysical profiling with simultaneous analysis of multiple immune cell markers. This platform allows to reveal notable cell type-dependent remodelling of plasma membrane order in diseases. By sorting immune cells based on their membrane order and performing functional and transcriptomic analyses, we link the plasma membrane order with cell states and reveal the underling transcriptomic codes. Our findings demonstrate a broad heterogeneity of plasma membrane order in immune cells that will provide a more precise definition of immune cell states based on their biophysical properties in health and disease.
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