Imaging Giardia intestinalis cellular organisation using expansion microscopy revealed atypical centrin localisation.
Soukup, J.; Zelena, M.; Weisz, F.; Kostelanska, M.; Nohynkova, E.; Tumova, P.
Show abstract
Advanced imaging of microorganisms, including protists, is challenging due to their small size. Specimen expansion prior to imaging is thus beneficial to increase resolution and cellular details. Here, we present a sample preparation workflow for improved observations of the single-celled eukaryotic pathogen Giardia intestinalis (Excavata, Metamonada). The binucleated trophozoites colonize the small intestine of humans and animals and cause a diarrhoeal disease. Their remarkable morphology includes two nuclei and a pronounced microtubular cytoskeleton enabling cell motility, attachment and proliferation. By use of expansion and confocal microscopy, we resolved in a great detail subcellular structures and organelles of the parasite cell. The acquired spatial resolution of 43 nm enabled novel observations of centrin localisation at Giardia basal bodies. Interestingly, non-luminal centrin localization between the Giardia basal bodies was observed, which is an atypical eukaryotic arrangement. Our protocol includes antibody staining and can be used for the localisation of epitope-tagged proteins, as well as for differential organelle labelling by amino reactive esters. This fast and simple protocol is suitable for routine use without a superresolution microscopy equipment.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- An extended DNA-free intranuclear compartment organizes centrosomal microtubules in Plasmodium falciparum 94%
- Kinesin-8B controls basal body function and flagellum formation and is key to malaria parasite transmission 92%
- Divergent Plasmodium protein kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesis 92%
Similar papers in this journal
- Nuclear pore complexes undergo Nup221 exchange during blood stage asexual replication of Plasmodium parasites. 93%
- Distribution of malaria parasite-derived phosphatidylcholine in the infected erythrocyte 90%
- Three-dimensional observations of an aperiodic oscillatory gliding motility behaviour in Myxococcus xanthus using confocal interference reflection microscopy 90%
Similar papers in this journal
- Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts 93%
- Systematic analysis of Plasmodium myosins reveals differential expression, localization and function in invasive and proliferative parasite stages 92%
- Vital role for Plasmodium berghei Kinesin8B in axoneme assembly during male gamete formation and mosquito transmission 91%
Similar papers in this journal
- The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei 94%
- Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes 94%
- Altered N-glycan composition impacts flagella mediated adhesion in Chlamydomonas reinhardtii 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.