Microvilli regulate the release modes of alpha-tectorin to organize the domain-specific matrix architecture of the tectorial membrane.
Niazi, A.; Kim, J. A.; Kim, D.-K.; Lu, D.; Sterin, I.; Park, J.; Park, S.
Show abstract
The tectorial membrane (TM) is an apical extracellular matrix (ECM) in the cochlea essential for auditory transduction. The TM exhibits highly ordered domain-specific architecture. Alpha-tectorin/TECTA is a glycosylphosphatidylinositol (GPI)-anchored ECM protein essential for TM organization. Here, we identified that TECTA is released by distinct modes: proteolytic shedding by TMPRSS2 and GPI-anchor-dependent release from the microvillus tip. In the medial/limbal domain, proteolytically shed TECTA forms dense fibers. In the lateral/body domain produced by the supporting cells displaying dense microvilli, the proteolytic shedding restricts TECTA to the microvillus tip and compartmentalizes the collagen-binding site. The tip-localized TECTA, in turn, is released in a GPI-anchor-dependent manner to form collagen-crosslinking fibers, required for maintaining the spacing and parallel organization of collagen fibrils. Overall, we showed that distinct release modes of TECTA determine the domain-specific organization pattern, and the microvillus coordinates the release modes along its membrane to organize the higher-order ECM architecture. O_FIG O_LINKSMALLFIG WIDTH=146 HEIGHT=200 SRC="FIGDIR/small/574255v1_ufig1.gif" ALT="Figure 1"> View larger version (36K): org.highwire.dtl.DTLVardef@10479c6org.highwire.dtl.DTLVardef@d6cb33org.highwire.dtl.DTLVardef@1b6b4fdorg.highwire.dtl.DTLVardef@2477f4_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOGraphical AbstractC_FLOATNO C_FIG
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis 96%
- Neural crest cells bulldoze through the microenvironment using Aquaporin-1 to stabilize filopodia 95%
- Ontogenesis of the tear drainage system requires Prickle 1-controlled polarized basement membrane (BM) deposition 95%
Similar papers in this journal
- A nesprin-4/kinesin-1 cargo model for nuclear positioning in cochlear outer hair cells 95%
- A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development 94%
- Chloride intracellular channel (CLIC) protein function in S1P-induced Rac1 activation requires membrane localization of the C-terminus, but not thiol-transferase nor ion channel activities. 94%
Similar papers in this journal
- Vertebrate extracellular matrix protein hemicentin-1 interacts physically and genetically with basement membrane protein nidogen-2 94%
- Human mammary 3D spheroid models uncover the role of filopodia in breaching the basement membrane to facilitate invasion 93%
- An SNP variant MT1-MMP with a defect in its collagenolytic activity confers the fibrotic phenotype of Dupuytren's Disease 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.