Back

Straightforward semi-quantitative MALDI-TOF MS based screening approach for selection of recombinant protein producing E. Coli

Kravtsov, I. N.; Solovyev, A. I.; Potemkina, E. A.; Kartashova, A. V.; Dmitrieva, M. A.; Danilova, K. V.; Tutykhina, I. L.; Polyakov, N. B.; Egorova, D. A.

2024-01-01 bioengineering
10.1101/2023.12.31.573754 bioRxiv
Show abstract

MALDI-TOF MS represents a rapid and cost-effective method for identifying proteins and microorganisms. When obtaining recombinant protein producers, differences in expression levels among transformants necessitate the conduction of a small-scale screening of expression levels. This study proposes a fast and easy method for screening clones producing recombinant proteins using MALDI-TOF MS. Various recombinant proteins were utilized to test the proposed method

Matching journals

The top 6 journals account for 50% of the predicted probability mass.

1
PLOS ONE
● 5266 papers in training set
Top 9%
19.2%
2
New Biotechnology
○ 12 papers in training set
Top 0.1%
12.4%
3
Applied Microbiology and Biotechnology
○ 32 papers in training set
Top 0.1%
5.7%
4
Frontiers in Bioengineering and Biotechnology
○ 98 papers in training set
Top 0.2%
5.7%
5
Scientific Reports
○ 3612 papers in training set
Top 22%
4.5%
6
Journal of Biotechnology
○ 11 papers in training set
Top 0.1%
3.4%
50% of probability mass above
7
International Journal of Molecular Sciences
○ 494 papers in training set
Top 3%
3.4%
8
Frontiers in Microbiology
○ 427 papers in training set
Top 4%
2.9%
9
Biotechnology and Bioengineering
○ 53 papers in training set
Top 0.3%
2.9%
10
International Journal of Biological Macromolecules
○ 76 papers in training set
Top 0.7%
2.2%
11
Virology
○ 61 papers in training set
Top 0.6%
2.0%
12
Methods
○ 34 papers in training set
Top 0.3%
1.6%
13
Biosensors and Bioelectronics
○ 57 papers in training set
Top 0.5%
1.4%
14
Journal of Immunological Methods
○ 24 papers in training set
Top 0.2%
1.4%
15
PeerJ
◐ 308 papers in training set
Top 7%
1.2%
16
Analytical and Bioanalytical Chemistry
○ 18 papers in training set
Top 0.2%
1.2%
17
ACS Omega
● 105 papers in training set
Top 3%
1.1%
18
Bioscience Reports
● 27 papers in training set
Top 1%
1.1%
19
Frontiers in Molecular Biosciences
○ 102 papers in training set
Top 2%
1.1%
20
Analytical Biochemistry
○ 26 papers in training set
Top 0.4%
1.0%
21
Sensors
○ 43 papers in training set
Top 1%
1.0%
22
The Analyst
● 16 papers in training set
Top 0.3%
0.9%
23
BioTechniques
○ 25 papers in training set
Top 0.4%
0.9%
24
Protein Expression and Purification
○ 13 papers in training set
Top 0.1%
0.6%
25
Biochimie
○ 25 papers in training set
Top 0.8%
0.6%
26
Food Microbiology
○ 11 papers in training set
Top 0.2%
0.6%
27
Analytical Chemistry
● 218 papers in training set
Top 2%
0.6%
28
BMC Biotechnology
○ 14 papers in training set
Top 0.3%
0.5%
29
Heliyon
○ 152 papers in training set
Top 10%
0.5%
30
Algal Research
○ 21 papers in training set
Top 0.5%
0.5%