A highly contiguous hexaploid wheat genome assembly facilitates analysis of 1RS translocation and mining of a new adult plant resistance locus to yellow rust disease
Li, G.; Ren, Y.; Yang, Y.; Chen, S.; Zheng, J.; Zhang, X.; Chen, M.; Sun, X.; Lv, C.; Li, X.; Li, Y.; Dong, C.; Tang, J.; Huang, Z.; Peng, Y.; Gu, D.; Wang, Z.; Zheng, H.; Shi, C.; Kang, G.; Zheng, T.; Chen, F.; Wang, D.; Zhang, K.; Yin, G.
Show abstract
High-quality genome information is essential for efficiently deciphering and improving crop traits. Here we report a highly contiguous hexaploid genome assembly for the key wheat breeding parent Zhou8425B, an elite 1BL/1RS translocation line with durable adult plant resistance (APR) against rust diseases. By using HiFi and Hi-C sequencing reads, a 14.75 Gb genome assembly, with contig N50 and scaffold N50 values reaching 70.94 and 735.11 Mb, respectively, was developed. Comparison with 16 previously sequenced common wheat cultivars revealed unique chromosomal structural features in Zhou8425B. Notably, the 1RS translocation in Zhou8425B was apparently longer and carried more genes encoding AP2/ERF-ERF and B3 transcription factors relative to its counterpart in several genome sequenced 1BL/1RS varieties and rye lines. Aided by Zhou8425B genome assembly, a new APR locus (i.e., YrZH3B) against yellow rust (YR) disease was finely mapped to a 1 - 2 Mb interval on chromosome 3BS. Analysis with 212 Zhou8425B derivative varieties showed that pyramiding of YrZH3B with two other APR loci (YrZH22 and YrZH84) significantly decreased YR severity and enhanced grain yield, with triple combination (YrZH3B/YrZH22/YrZH84) having the highest effects. Our data demonstrate the high value of Zhou8425B assembly in studying wheat genome and agronomically important genes.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases 95%
- Structural variations contribute to subspeciation and yield heterosis in rice 95%
- Thinopyrum intermedium TiAP1 interacts with a chitin deacetylase from Blumeria graminis f. sp. tritici and increases the resistance to Bgt in wheat 95%
Similar papers in this journal
- Boosting wheat functional genomics via indexed EMS mutant library of KN9204 97%
- A near-complete genome sequence of einkorn wheat provides insight into the evolution of wheat A subgenomes 96%
- Transcriptome brings variations of gene expression, alternative splicing, and structural variations into gene-scale trait dissection in soybean 96%
Similar papers in this journal
- ALI-1, candidate gene of B1 locus, is associated with awn length and grain weight in common wheat 97%
- Degenerate oligonucleotide primer MIG-seq: an effective PCR-based method for high-throughput genotyping 96%
- Effects of homoeologous exchange on gene expression and alternative splicing in a newly formed allotetraploid wheat 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.