ROS-induced translational regulation--through spatiotemporal differences in codon recognition--is a key driver of brown adipogenesis
Ip, J. Y.; Wijaya, I.; Lee, L. T.; Lim, Y.; Teoh, D. E.-J.; Chan, C. S. C.; Cui, L.; Begley, T. J.; Dedon, P. C.; Guo, H.
Show abstract
The role of translational regulation in brown adipogenesis is relatively unknown. Localized translation of mRNAs encoding mitochondrial components enables swift mitochondrial responses, but whether this occurs during brown adipogenesis, which involves massive mitochondrial biogenesis, has not been explored. Here, we used ribosome profiling and RNA-Seq, coupled with cellular fractionation, to obtain spatiotemporal insights into translational regulation. During brown adipogenesis, a translation bias towards G/C-ending codons is triggered first in the mitochondrial vicinity by reactive oxygen species (ROS), which later spreads to the rest of the cell. This translation bias is induced through ROS modulating the activity of the tRNA modification enzyme, ELP3. Intriguingly, functionally relevant mRNAs, including those encoding ROS scavengers, benefit from this bias; in so doing, ROS-induced translation bias both fuels differentiation and concurrently minimizes oxidative damage. These ROS-induced changes could enable sustained mitochondrial biogenesis during brown adipogenesis, and explain in part, the molecular basis for ROS hormesis.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The master energy homeostasis regulator PGC-1alpha couples transcriptional co-activation and mRNA nuclear export 96%
- Mitochondrial membrane potential regulates nuclear DNA methylation and gene expression through phospholipid remodeling 95%
- Thioesterase Superfamily Member 1 Undergoes Stimulus-coupled Reorganization to Regulate Metabolism 95%
Similar papers in this journal
- eIF3 engages with 3'-UTR termini of highly translated mRNAs 95%
- Robust single nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity 95%
- A mouse model of human mitofusin 2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion 94%
Similar papers in this journal
- Cold-induced expression of a truncated Adenylyl Cyclase 3 acts as rheostat to brown fat function. 96%
- Dihydroxyacetone phosphate signals glucose availability to mTORC1 95%
- Short-chain fatty acid metabolites propionate and butyrate are unique epigenetic regulatory elements linking diet, metabolism and gene expression 95%
Similar papers in this journal
- Small mitochondrial protein NERCLIN regulates cardiolipin homeostasis and mitochondrial ultrastructure. 95%
- Coordination of rhythmic RNA synthesis and degradation orchestrates 24-hour and 12-hour RNA expression patterns in mouse fibroblasts 94%
- Conserved 5-methyluridine tRNA modification modulates ribosome translocation 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.