The membrane curvature inducing REEP1 proteins generate a novel ER-derived vesicular compartment
Shibata, Y.; Mazur, E. E.; Pan, B.; Hernandez, S. V.; Zhang, J.; Rapoport, T. A.
Show abstract
The endoplasmic reticulum (ER) is shaped by abundant, membrane curvature-generating proteins that include the REEP family member REEP5. The REEP1 subfamily, consisting of REEP1-4 in mammals, differs in abundance and topology from REEP5. Mutations in REEP1 and REEP2 cause Hereditary Spastic Paraplegia, but REEP1-4s function remains enigmatic. Here we show that the REEP1 proteins reside in a novel vesicular compartment and identify features that determine their localization. Mutations in REEP1 proteins that compromise curvature-inducing activity, including those that cause disease, relocalize the proteins to the bulk ER. These mutants interact with wildtype proteins to retain them in the ER, consistent with their autosomal-dominant disease inheritance. REEP1vesicles contain the fusogen atlastin-1, but not general ER proteins. We propose that REEP1 proteins generate these vesicles themselves by budding directly from the ER, and that they cycle back to the ER by atlastin-mediated fusion. The vesicles may serve to regulate ER tubule dynamics.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope 97%
- Identification of two pathways mediating protein targeting from ER to lipid droplets 96%
- Lysosome damage triggers acute formation of ER to lysosomes membrane tethers mediated by the bridge-like lipid transport protein VPS13C 95%
Similar papers in this journal
- C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin 97%
- The ORP9-ORP11 dimer promotes sphingomyelin synthesis 96%
- Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.