Staphylococcus aureus can use an alternative pathway to be internalized by osteoblasts in absence of β1 integrins
Mouton, W.; Tricou, L.-P.; Cara, A.; Trouillet-Assant, S.; Bouvard, D.; Laurent, F.; Diot, A.; Josse, J.
Show abstract
Staphylococcus aureus main internalization mechanism in osteoblasts relies on a tripartite interaction between bacterial fibronectin-binding proteins, extracellular matrix soluble fibronectin, and osteoblasts {beta}1 integrins. Caveolins, and particularly caveolin-1, have shown to limit the plasma membrane microdomain mobility, and consequently reduce the uptake of S. aureus in keratinocytes. In this study, we aimed to deepen our understanding of the molecular mechanisms underlying S. aureus internalization in osteoblasts. Mechanistically, S. aureus internalization requires endosomal recycling {beta}1 integrins as well as downstream effectors such as Src, Rac1, and PAK1. Surprisingly, in {beta}1 integrin deficient osteoblasts, S. aureus internalization is restored when Caveolin-1 is absent and requires v{beta}3/v{beta}5 integrins as backup fibronectin receptors. Altogether, our data support that {beta}1 integrins regulate the level of detergent-resistant membrane at the plasma membrane in a an endosomal and Caveolin-1 dependent manner. SUMMARY STATEMENTStaphylococcus aureus can be internalized by osteoblasts via a different mechanism than the main 5{beta}1/fibronectin/fibronectin-binding protein that likely involves v{beta}3 or v{beta}5 integrin.
Matching journals
The top 11 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Investigating pathogenicity and virulence of Staphylococcus pettenkoferi: an emerging pathogen 91%
- NDR2 Kinase Regulate Microglial Metabolic Adaptation and Inflammatory Response: Critical Role in Glucose-Dependent Functional Plasticity 91%
- Hematopoietic growth factors Regulate Entry of Monocytes into the Adult Brain via Chemokine Receptor CCR5 90%
Similar papers in this journal
- A1 Adenosine Receptor Signaling Reduces Streptococcus pneumoniae Adherence to Pulmonary Epithelial Cells by Targeting Expression of Platelet-Activating Factor Receptor 92%
- SopB- and SifA-dependent shaping of the Salmonella-containing vacuole proteome in the social amoeba Dictyosteliumdiscoideum 91%
- Fluorescent protein-based reporters reveal stress response of intracellular Salmonella enterica on single cell level 90%
Similar papers in this journal
- Sensitizing Staphylococcus aureus to antibacterial host defense by decoding and blocking the lipid flippase MprF 94%
- Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius. 91%
- Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.