Glioma Cells Secrete Collagen VI to Facilitate Invasion
Cha, J.; Ding, E. A.; Carvalho, E. M.; Fowler, A.; Aghi, M. K.; Kumar, S.
Show abstract
While glioblastoma (GBM) progression is associated with extensive extracellular matrix (ECM) secretion, the causal contributions of ECM secretion to invasion remain unclear. Here we investigate these contributions by combining engineered materials, proteomics, analysis of patient data, and a model of bevacizumab-resistant GBM. We find that GBM cells cultured in engineered 3D hyaluronic acid hydrogels secrete ECM prior to invasion, particularly in the absence of exogenous ECM ligands. Proteomic measurements reveal extensive secretion of collagen VI, and collagen VI-associated transcripts are correspondingly enriched in microvascular proliferation regions of human GBMs. We further show that bevacizumab-resistant GBM cells deposit more collagen VI than their responsive counterparts, which is associated with marked cell-ECM stiffening. COL6A3 deletion in GBM cells reduces invasion, {beta}-catenin signaling, and expression of mesenchymal markers, and these effects are amplified in hypoxia. Our studies strongly implicate GBM cell-derived collagen VI in microenvironmental remodeling to facilitate invasion.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Mechanical intercellular communication via matrix-borne cell force transmission during vascular network formation 95%
- Biomechanical Phenotyping Reveals Unique Mechanobiological Signatures of Early-Onset Colorectal Cancer 94%
- Mechanical environment afforded by engineered polymer hydrogel critically regulates survival of neural stem cells transplanted in the injured spinal cord via Piezo1-mediated mechanotransduction 94%
Similar papers in this journal
- Dormancy-inducing 3D-engineered matrix uncovers mechanosensitive and drug protective FHL2-p21 signaling axis 94%
- Xenogeneic Skin Transplantation Promotes Angiogenesis and Tissue Regeneration Through Vitamin D-Activated Trem2+ Macrophages 94%
- Immunotherapy of glioblastoma explants induces interferon-γ responses and immune cell rearrangements in tumor center, but not periphery 94%
Similar papers in this journal
- Gels for Live Analysis of Compartmentalized Environments (GLAnCE): A Tissue Model to Probe Tumour Phenotypes at Tumour-Stroma Interfaces 95%
- Bone mineral density affects tumor growth by shaping microenvironmental heterogeneity 93%
- Soluble ECM promotes organotypic formation in lung alveolar model 92%
Similar papers in this journal
- Membrane to cortex attachment determines different mechanical phenotypes in LGR5+ and LGR5- colorectal cancer cells 94%
- Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments 93%
- Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype 93%
Similar papers in this journal
- Monocytes use protrusive forces to generate migration paths in viscoelastic collagen-based extracellular matrices 93%
- Mechanophenotyping of 3D Multicellular Clusters using Displacement Arrays of Rendered Tractions 93%
- Murine gut microbiota dysbiosis via enteric infection modulates the foreign body response to a distal biomaterial implant 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.