Postnatal FGF-signalling establishes gradients of cell-identities along the proximal-distal axis of the lung airways
Sountoulidis, A.; Firsova, A. B.; Liontos, A.; Theelke, J.; Koepke, J.; Millar-Buchner, P.; Manneras-Holm, L.; Bjorklund, A. K.; Fysikopoulos, A.; Gaengel, K.; Backhed, F.; Betsholtz, C.; Seeger, W.; Bellusci, S.; Samakovlis, C.
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Secretory cells are major structural and functional constituents of the lung airways. Their spatial organization and specification mechanisms are partially understood. Here, we labelled major secretory airway cell types and analysed them at single-cell resolution. We found opposing, partially overlapping gene-expression gradients along the proximal-distal airway axis superimposed on a general gene program encoding detoxification. One graded program is elevated proximally and relates to innate immunity, whereas the other is enriched distally, encoding lipid metabolism and antigen presentation. Intermediately positioned cells express low levels of both graded programs and show increased clonogenic potency in vitro, relating cell-plasticity to location in each branch. Single-cell RNA-sequencing following lineage-tracing revealed the sequential and postnatal establishment of the gradients in common epithelial progenitors. Fgfr2b is distally enriched and its postnatal inactivation reduces distal gene expression and expands proximal genes into distally located cells. This suggests a central role of FGFR-signaling in tissue-scale airway patterning.
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