The Arabidopsis PPR protein EMB1006 interacts with both EMB1270 and CFM2 to facilitate plastid clpP1 intron2 splicing
Zhang, L.; Zhu, Y.; Yang, K.; Liao, F.; Wang, J.; Zhou, S.; Huang, J.; Cui, Y.-L.; Huang, W.
Show abstract
Plastid intron splicing is an essential step for gene expression. Although many nucleus-encoded splicing factors have been identified for plastid intron splicing, how these proteins coordinately regulate intron splicing is unclear. Here we found that EMB1006, an Arabidopsis P-type PPR protein, is required for splicing several plastid introns including clpP1 intron 2 by analyzing the level of intron retention in its knockdown lines and RIP-qPCR (RNA immunoprecipitation and quantitative PCR) assay. In addition, RNA electrophoretic mobility shift assay (REMSA) showed that EMB1006 specifically binds to the sequence UUACCAAACGU close to the 3 end of clpP1 exon 2 in vitro. Furthermore, yeast two hybrid (Y2H), split luciferase complementation (Split-LUC) and semi-in vivo pull-down assays showed that EMB1006 interacts with both EMB1270 and CFM2, which also interact each other. Taken together, our data indicate that EMB1006 forms a complex with EMB1270 and CFM2 to facilitate clpP1 intron 2 splicing in chloroplasts.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- DROL1 subunit of U5 snRNP in the spliceosome is specifically required to splice AT--AC-type introns in Arabidopsis 97%
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2 96%
- FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface 96%
Similar papers in this journal
- SlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato 96%
- A network of RS splicing regulatory proteins controls light-dependent splicing and seedling development 96%
- Disruption of the mitochondrial gene orf352 partially restores pollen development in cytoplasmic male sterile rice 95%
Similar papers in this journal
- Tomato SlBES1.8 influences leaf morphogenesis by mediating gibberellin metabolism and signaling 96%
- Transcriptomic dynamics of petal development in the one-day flower species, Japanese morning glory (Ipomoea nil) 95%
- DLDG1, the Arabidopsis Homolog of the Cyanobacterial H+-Extrusion Protein, Regulates Non-Photochemical Quenching in Chloroplasts 95%
Similar papers in this journal
- Arabidopsis ERF4 and MYB52 Transcription Factors Play Antagonistic Roles in Regulating Homogalacturonan De-methylesterification in Seed Coat Mucilage 96%
- Verticillium dahliae effector VDAL protects MYB6 from degradation by interacting with PUB25/26 E3 ligases for enhancing Verticillium wilt resistance in Arabidopsis 96%
- Correction of frameshift mutations in the atpB gene by translational recoding in chloroplasts of Oenothera and tobacco. 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.