Influenza infected macrophages release viral ribonucleoproteins that shape the local host response
Fritzlar, S.; Lakdawala, S.; Roberts, J. A.; Coin, L. J. M.; Deng, Y.-M.; Moselen, J.; Le Sage, V.; Mackenzie, J.; Labzin, L.; Brooks, A. G.; Reading, P.; Londrigan, S.
Show abstract
Airway epithelial cells and macrophages (M{Phi}) represent cellular targets of infection by influenza A virus (IAV). Epithelial cells support IAV infection and replication by enabling the generation and release of new viral particles (productive replication). In contrast, M{Phi} are susceptible to initial IAV infection but the release of infectious viral particles is inhibited through abortive replication. Despite the lack of infectious virions released from infected M{Phi}, we detected newly synthesised viral RNA and nucleoprotein (NP) in M{Phi} supernatants. We show that viral RNA is released from infected M{Phi} as viral ribonucleoprotein (vRNP) complexes which elicits potent inflammatory responses when exposed to uninfected cells. These vRNPs specifically induced IL-1{beta}, CXCL13, IL-32, CCL4 and CXCL10 in uninfected cells and were at least partially sensed through the RIG-I/MDA5 pathway. While M{Phi} represent a dead-end for IAV infection through abortive replication, the release of vRNPs shapes immune repsonses of uninfected cells in the local microenvironment.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Respiratory syncytial virus sequesters NF-κB subunit p65 to cytoplasmic inclusion bodies to inhibit innate immune signalling 96%
- HA stability regulates H1N1 influenza virus replication and pathogenicity in mice by modulating type I interferon responses in dendritic cells 95%
- Human nasal and lung tissues infected ex vivo with SARS-CoV-2 provide insights into differential tissue-specific and virus-specific innate immune responses in the upper and lower respiratory tract 95%
Similar papers in this journal
- Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly. 96%
- Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking 95%
- The astrovirus N-terminal nonstructural protein anchors replication complexes to the perinuclear ER membranes 95%
Similar papers in this journal
- The genetic driver of Acute Necrotizing Encephalopathy, RANBP2, regulates the inflammatory response to Influenza A virus infection 96%
- Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology 95%
- Omicron Spike confers enhanced infectivity and interferon resistance to SARS-CoV-2 in human nasal tissue 95%
Similar papers in this journal
- Y-box binding protein 1 interacts with dengue virus nucleocapsid and mediates viral assembly 95%
- Comparison of SARS-CoV-2 variants of concern in primary human nasal cultures demonstrates Delta as most cytopathic and Omicron as fastest replicating 95%
- Essential functions of Inositol hexakisphosphate (IP6) in Murine Leukemia Virus replication 94%
Similar papers in this journal
- TMPRSS2 is essential for SARS-CoV-2 Beta and Omicron infection 94%
- Chandipura Virus forms cytoplasmic inclusion bodies through phase separation and proviral association of cellular protein Kinase R and stress granules protein TIA-1 94%
- Betacoronaviruses Differentially Activate the Integrated Stress Response to Optimize Viral Replication in Lung Derived Cell Lines 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.