A modified RGB trichrome (HemRGB) for improving nuclear staining: application to colorectal cancer histopathology
Gaytan, F.
Show abstract
The RGB trichrome staining method has been used to highlight two major components of the extracellular matrix, collagen and glycosaminoglycans. While the RGB trichrome efficiently stains extracellular matrix components, it lacks a nuclear stain, limiting its application in histopathology. To address this issue, a modification of the original stain, named HemRGB trichrome, has been developed. This modification incorporates iron hematoxylin for improving nuclear staining while retaining specificity for the staining of extracellular matrix. The application of HemRGB trichrome staining to samples from both normal colonic tissues and colorectal adenocarcinomas (CRC) provides a robust nuclear staining, together with a high-contrasted staining of tumor microenvironmental components, such as infiltrating immune cells, collagen and ground substance, extracellular mucins, as well as contrasted interfaces between CRC metastases and liver parenchyma. This study underscores the potential of HemRGB trichrome as a valuable tool for histopathological studies, especially for cancer evaluation, where nuclear characteristics are particularly relevant.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- TMIGD1, a putative tumor suppressor, induces G2-M cell cycle checkpoint arrest in colon cancer cells 93%
- Role of HNF4alpha-cMyc Interaction in CDE-diet Induced Liver Injury and Regeneration 92%
- Detection of Colorectal Adenocarcinoma and Grading Dysplasia on Histopathologic Slides Using Deep Learning 92%
Similar papers in this journal
- A digital score of peri-epithelial lymphocytic activity predicts malignant transformation in oral epithelial dysplasia 93%
- Spatial Effects of Infiltrating T cells on Neighbouring Cancer Cells and Prognosis in Stage III CRC patients 92%
- The hepatocyte Epidermal Growth Factor Receptor (EGFR) pathway regulates the cellular interactome within the liver fibrotic niche 90%
Similar papers in this journal
- Comparative Immune profiling in Pancreatic Ductal Adenocarcinoma Progression Among South African patients 94%
- PDAC-ANN: an artificial neural network to predict Pancreatic Ductal Adenocarcinoma based on gene expression 93%
- The Concept of Stroma AReactive Invasion Front Areas (SARIFA) as a New Prognostic Biomarker for Lipid-driven Cancers Holds True in Pancreatic Ductal Adenocarcinoma 93%
Similar papers in this journal
- Prostate Specific Membrane Antigen expression in the vasculature of primary lung carcinomas associates with faster metastatic dissemination to brain 91%
- The transition zone in Hirschsprung’s bowel contains abnormal hybrid ganglia with characteristics of extrinsic nerves 91%
- Dendritic cell-derived lncRNA in patients with acute coronary syndrome 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.