The antiviral protein Shiftless blocks p-body formation during KSHV infection.
Hatfield, D.; Rodriguez, W.; Mehrmann, T.; Muller, M.
Show abstract
P-bodies (PB) are non-membranous foci involved in determining mRNA fate by affecting translation and mRNA decay. In this study, we identify the anti-viral factor SHFL as a potent disassembly factor of PB. We show that PBs remain sparse in the presence of SHFL even in the context of oxidative stress, a major trigger for PB induction. Mutational approaches revealed that SHFL RNA binding activity is not required for its PB disassembly function. However, we have identified a new region of SHFL which bridges two distant domains as responsible for PB disassembly. Furthermore, we show that SHFL ability to disrupt PB formation is directly linked to its anti-viral activity during KSHV infection. While WT SHFL efficiently restricts KSHV lytic cycle, PB disruption defective mutants no longer lead to reactivation defects. SHFL-mediated PB disassembly also leads to increased expression of key anti-viral cytokines, further expanding SHFL dependent anti-viral state. Taken together, our observations suggest a role of SHFL in PB disassembly, which could have important anti-viral consequences during infection.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- LANA-Dependent Transcription-Replication Conflicts and R-Loops at the Terminal Repeats (TR) Correlate with KSHV Episome Maintenance 96%
- BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection 96%
- Characterisation of the Semliki Forest Virus-host cell interactome reveals the viral capsid protein as an inhibitor of nonsense-mediated mRNA decay 95%
Similar papers in this journal
- The m6A reader YTHDC2 is essential for escape from KSHV SOX-induced RNA decay 97%
- Pseudouridine prevalence in Kaposi's sarcoma associated herpesvirus transcriptome reveals an essential mechanism for viral replication 96%
- The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomes 95%
Similar papers in this journal
- The genetic driver of Acute Necrotizing Encephalopathy, RANBP2, regulates the inflammatory response to Influenza A virus infection 96%
- Identification of DAXX As A Restriction Factor Of SARS-CoV-2 Through A CRISPR/Cas9 Screen 95%
- PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription 95%
Similar papers in this journal
- Dynamic interactions of retroviral Gag condensates with nascent viral RNA at transcriptional burst sites: implications for genomic RNA packaging 96%
- Interferon-responsive genes are targeted during the establishment of human cytomegalovirus latency 95%
- Caspase-mediated regulation and cellular heterogeneity of the cGAS/STING pathway in Kaposi's sarcoma-associated herpesvirus infection 95%
Similar papers in this journal
- The N-terminal and central domains of CoV-2 nsp1 play key functional roles in suppression of cellular gene expression and preservation of viral gene expression 96%
- Viral E Protein Neutralizes BET Protein-Mediated Post-Entry Antagonism of SARS-CoV-2 95%
- Temporal Dynamics of HCMV Gene Expression in Lytic and Latent Infections 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.