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scLongTree: an accurate computational tool to infer the longitudinal tree for scDNAseq data

Khan, R.; Mallory, X.

2023-11-15 bioinformatics
10.1101/2023.11.11.566680 bioRxiv
Show abstract

A subclonal tree that depicts the evolution of cancer cells is of interest in understanding how cancer grows, prognosis and treatment of cancer. Longitudinal single-cell DNA sequencing data (scDNA-seq) is the single-cell DNA sequencing data sequenced at different time points. It provides more knowledge of the order of the mutations than the scDNA-seq taken at only one time point, and thus facilitates the inference of the subclonal tree. There is only one existing tool LACE that can infer a subclonal tree based on the longitudinal scDNA-seq, and it is limited in accuracy and scale. We presented scLongTree, a computational tool that can accurately infer the longitudinal subclonal tree based on the longitudinal scDNA-seq. ScLongTree can be scalable to hundreds of mutations, and outper-formed state-of-the-art methods SCITE, SiCloneFit and LACE on a comprehensive simulated dataset. The test on a real dataset SA501 showed that scLongTree can more accurately interpret the progres-sive growth of the tumor than LACE. ScLongTree is freely available on https://github.com/compbio-mallory/sc longitudinal infer.

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