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Tick extracellular vesicles impair epidermal homeostasis through immune-epithelial networks during hematophagy

Marnin, L.; Bogale, H.; Laukaitis-Yousey, H.; Valencia, L.; Rolandelli, A.; O'Neal, A.; Ferraz, C.; Schmitter-Sanchez, A.; Bencosme Cuevas, E.; Nguyen, T.-T.; Leal-Galvan, B.; Rickert, D.; Bruno, V. M.; Mendes, M. T.; Samaddar, S.; Butler, L. R.; Singh, N.; Cabrera Paz, F.; Oliver, J.; Jameson, J.; Munderloh, U.; Oliva Chavez, A.; Mulenga, A.; Park, S.; Serre, D.; Pedra, J. H. F.

2023-11-10 microbiology Community evaluation
10.1101/2023.11.10.566612 bioRxiv
Show abstract

Wound healing has been extensively studied through the lens of inflammatory disorders and cancer, but limited attention has been given to hematophagy and arthropod-borne diseases. Hematophagous ectoparasites, including ticks, subvert the wound healing response to maintain prolonged attachment and facilitate blood-feeding. Here, we unveil a strategy by which extracellular vesicles (EVs) ensure blood-feeding and arthropod survival in three medically relevant tick species. Through single cell RNA sequencing and murine genetics, we demonstrate that wildtype animals infested with EV-deficient Ixodes scapularis display a unique epidermal sub-population with a mesenchymal-like transcriptional program and an overrepresentation of pathways connected to wound healing. Furthermore, tick EVs inhibit proliferation and diminish the capacity of wound closure in keratinocytes. This occurrence was linked to phosphoinositide 3-kinase activity, keratinocyte growth factor 1 (KGF-1) and transforming growth factor {beta} (TGF-{beta}) levels. Collectively, we uncovered a strategy employed by a blood-feeding arthropod that disrupts the circuitry in cutaneous wound healing, contributing to ectoparasite fitness.

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