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Pauperization of Emerin from nuclear envelope drives prostate cancer cell migration and invasiveness

Nastaly, P.; Popeda, M.; Kowalski, K.; Wenta, T.; Beznoussenko, G.; Rychlowski, M.; Mironov, A.; Lavagnino, Z.; Barozzi, S.; Richert, J.; Bertolio, R.; Szade, J.; Miszewski, K.; Matuszewski, M.; Zaczek, A. J.; Braga, L.; Del Sal, G.; Bednarz-Knoll, N.; Maiuri, P.

2023-11-07 cancer biology
10.1101/2023.11.06.565767 bioRxiv
Show abstract

Micronuclei (MN) can arise from many causes, including the breakage of aberrant cytokinetic chromatin bridge. The frequent observation of MN in tumors raises the specter that they might not merely be passive elements but could instead play active roles in tumor progression. Here, we test the hypothesis that the presence of micronuclei could induce specific phenotypic and functional changes to the cell and lead to increased cancer invasive potential. With a variety of imaging and molecular methods in vitro and in clinical samples from prostate cancer (PCa) patients, we show that chromosome bridge resolution can lead to EMD accumulation and formation of EMD-rich MN. Such structure is negative for Lamin A/C and positive for LBR and Sec6{beta}. It can cause EMD pauperization from NE affecting migratory and invasive properties of a cell and can be translated to PCa patients poor prognosis.

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