High-throughput single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities.
Hira, J.; Singh, B.; Halder, T.; Mahmutovic, A.; Ajayi, C.; Sekh, A. A.; Hegstad, K.; Johannessen, M.; Lentz, C. S.
Show abstract
Isogenic bacterial cell populations are phenotypically heterogenous and may include subpopulations of antibiotic tolerant or heteroresistant cells. The reversible nature of these phenotypes and lack of biomarkers to differentiate functionally different, but morphologically identical cells is a challenge for research and clinical detection. To overcome this, we present Cellular Phenotypic Profiling and backTracing (CPPT), a flexible fluorescence-activated cell sorting platform, that uses optical probes to visualize and quantify cellular traits and connects the resulting phenotypic profile with a cells experimentally determined fate in single cell-derived growth and antibiotic susceptibility analysis. By applying CPPT on Staphylococcus aureus populations we recorded phenotypic signatures for dormant cells, exposed microanatomy-independent bimodal growth patterns in colony-derived cells, and revealed different culturability of single cells on solid compared to liquid media. We demonstrate that vancomycin-bodipyFL marks cellular subpopulations with increased likelihood to survive antibiotic exposure, showcasing the value of CPPT for discovery of clinically relevant biomarkers.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST 95%
- Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria 95%
- ColTapp, an automated image analysis application for efficient microbial colony growth dynamics quantification 94%
Similar papers in this journal
- Emergence of ion-channel mediated electrical oscillations in E. coli biofilms 94%
- Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq 94%
- Spatial integration of sensory input and motor output in Pseudomonas aeruginosa chemotaxis through colocalized distribution 94%
Similar papers in this journal
- Decoupling growth and production by removing the origin of replication from a bacterial chromosome 95%
- Protein sensors of bacterial kinase activity reveal antibiotic-dependent kinase activation in single cells 94%
- Engineering a novel probiotic toolkit in Escherichia coli Nissle1917 for sensing and mitigating gut inflammatory diseases 94%
Similar papers in this journal
- Pseudomonas aeruginosa and Candida albicans both accumulate greater biomass in dual species biofilms under flow 93%
- Human Saliva Modifies Growth, Biofilm Architecture and Competitive Behaviors of Oral Streptococci 93%
- Clostridioides difficile-mucus interactions encompass shifts in gene expression, metabolism, and biofilm formation 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.