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Deciphering the biology and chemistry of the mutualistic partnership between Bacillus velezensis and the arbuscular mycorrhizal fungus Rhizophagus irregularis

Anckaert, A.; Declerck, S.; Poussart, L.-A.; Lambert, S.; Helmus, C.; Boubsi, F.; Steels, S.; Arguelles Arias, A.; Calonne-Salmon, M.; ongena, m.

2023-10-28 microbiology
10.1101/2023.10.28.564539 bioRxiv
Show abstract

Arbuscular mycorrhizal (AM) fungi (e.g. Rhizophagus irregularis) recruit specific bacterial species in their hyphosphere. However, the chemical interplay and the mutual benefit of this intricate partnership have not yet been investigated especially as it involves bacteria known as strong producers of antifungal compounds such as Bacillus velezensis. Here, we show that the soil dwelling B. velezensis migrates along the hyphal network of the AM fungus R. irregularis, forming biofilms and inducing metabolic fluxes that contributes to host plant root colonization by the bacterium. During hyphosphere colonization, R. irregularis modulates the biosynthesis of specific lipopeptides and antimicrobial compounds in B. velezensis as a mechanism toward-off mycoparasitic fungi and bacteria to ensure stable coexistence. These mutual benefits are extended into a tripartite context via the provision of enhanced protection to the host plant through the induction of systemic resistance.

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