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Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein

Chan, S. H.; Tirole, L.; Kneller, D. W.; Kelley, T. M.; Dai, N.; Robb, G. B.

2023-10-28 biochemistry
10.1101/2023.10.28.564488 bioRxiv
Show abstract

mRNA vaccines and therapeutics are highly effective and can be developed and manufactured with a relatively short lead time. Here we report an all-enzyme platform to generate capped synthetic RNA in a one-step process based on an RNA capping enzyme-T7 RNA polymerase fusion protein. Under standard in vitro transcription reaction conditions, the fusion protein, in conjunction with an RNA cap 2'-O-methyltransferase, can generate synthetic mRNA with up to 95% of Cap-1 incorporation, greatly simplifying mRNA manufacturing workflows.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.