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Effective biotechnology for reducing N2O-emissions from farmland: N2O-respiring bacteria vectored by organic waste

Hiis, E. G.; Vick, S. H. W.; Molstad, L.; Rosdal, K.; Jonassen, K. R.; Winiwarter, W.; Bakken, L. R.

2023-10-19 microbiology
10.1101/2023.10.19.563143 bioRxiv
Show abstract

Farmed soils contribute to global warming primarily by N2O-emissions, and mitigation has proven difficult. However, a novel approach with promising results in the laboratory, exploits organic wastes both as substrates and vectors for strains of N2O-respiring bacteria (NRB), selected for their ability to survive in soil. Here we demonstrate a strong effect in field experiments: fertilization with waste from biogas-production, in which the strain Cloacibacterium sp. CB-01 had grown aerobically to [~]6*109 cells mL-1, reduced N2 O-emissions by 50-95 %. The strong and long-lasting effect of CB-01 is ascribed to its tenacity in soil, rather than its biokinetic parameters, which were inferior to other NRB-strains. Scaling up to EU level, we find that national anthropogenic N2O-emissions can be reduced by 5-20 %, and more if including other organic wastes. This opens an avenue for cost-effective reduction of N2O-emissions for which other mitigation options are currently lacking.

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