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Study on the preparation of bovine milk exosomes and the stability of lyophilized powder

Ge, X.; Li, T.; Lu, L.; Han, C.; Wang, M.; Du, H.; Chen, N.; Gao, M.; Wang, N.; Qi, D.; Bai, W.; Yin, J.; Dong, F.

2023-10-19 bioengineering
10.1101/2023.10.16.562624 bioRxiv
Show abstract

Bovune milk exosomes (MK-exo) have bioactive effects on the health of consumers and can also serve as excellent drug delivery vehicles. However, long-term preservation and transportation remain major challenges at present. In this report, high-quality MK-exo are prepared on a large scale. We then developed a lyophilization method belonging to MK-exo, and performed acceleration and long-term stability experiments on the lyophilized MK-exo to evaluate the effect of lyophilization. The results showed that the lyophilized MK-exo can be stored at room temperature for at least 3 months and 2-8{degrees}C for 15 months. These findings provide data support for further understanding of the properties and applications of MK-exo.

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