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FluxNorm: Toolbox for metabolic flux assay normalization by in situ cell counting

Djaja, N. A.; Bracha, T.; Yu, S. B.; Wang, H.; Carlson, N. M.; Pekkurnaz, G.

2023-10-14 cell biology
10.1101/2023.10.13.562314 bioRxiv
Show abstract

Plate-based quantitative metabolic flux analysis has emerged as the central technology to examine cellular metabolism and mitochondrial bioenergetics. However, accurate interpretation of metabolic activity between different experimental conditions in multi-well microplates requires data normalization based on in situ cell counts. Here, we describe FluxNorm, a platform-independent semi-automated computational workflow, validated for three different cell types, to normalize cell density for accurate assessment of cellular bioenergetics.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.