Integrator-mediated clustering of poised RNA polymerase II synchronizes histone transcription
Lu, F.; Park, B. J.; Fujiwara, R.; Wilusz, J. E.; Gilmour, D. S.; Lehmann, R.; Lionnet, T.
Show abstract
Numerous components of the transcription machinery, including RNA polymerase II (Pol II), accumulate in regions of high local concentration known as clusters, which are thought to facilitate transcription. Using the histone locus of Drosophila nurse cells as a model, we find that Pol II forms long-lived, transcriptionally poised clusters distinct from liquid droplets, which contain unbound and paused Pol II. Depletion of the Integrator complex endonuclease module, but not its phosphatase module or Pol II pausing factors disperses these Pol II clusters. Consequently, histone transcription fails to reach peak levels during S-phase and aberrantly continues throughout the cell cycle. We propose that Pol II clustering is a regulatory step occurring near promoters that limits rapid gene activation to defined times. One Sentence SummaryUsing the Drosophila histone locus as a model, we show that clustered RNA polymerase II is poised for synchronous activation.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Two pathways drive meiotic chromosome axis assembly in Saccharomyces cerevisiae 97%
- Safeguarding Genome Integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 Orchestrate Topoisomerase II-Mediated Catenane Resolution in Mitosis 97%
- Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions 97%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.