Dairy practices select for thermostable endolysins in phages
Oechslin, F.; Zhu, X.; Morency, C.; Somerville, V.; Shi, R.; Moineau, S.
Show abstract
Endolysins are phage lytic enzymes that degrade the bacterial cell wall to release phage progeny. We found that the diversity of endolysins is unsuspectedly low and dominated by a single structural type in distinct phages that infect Streptococcus thermophilus. Through X-ray crystallography, we discovered that this type of endolysin contains a highly conserved calcium-binding motif in its cell wall binding domain. Inactivation of the motif in the purified endolysin or in the phage genome revealed its key role in stabilizing the enzyme under conditions that mimic the cheesemaking process, including at elevated temperatures. Fermented dairy products such as yogurt and cheeses are incubated or cooked at higher temperatures and require the addition of thermophilic lactic acid bacteria such as S. thermophilus to drive milk fermentation. It appears that dairy practices have influenced the genetic signatures of streptococcal phages by selecting for thermostable endolysins, which in turn have limited their diversity.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Type I toxin-antitoxin systems contribute to mobile genetic elements maintenance in Clostridioides difficile and can be used as a counter-selectable marker for chromosomal manipulation 96%
- An ancient lysozyme in placozoans participates in acidic extracellular digestion 95%
- Quantitative proteome of bacterial periplasmic predation reveals a prey damaging protease 93%
Similar papers in this journal
- Strengthening Phage Resistance of Streptococcus thermophilus by Leveraging Complementary Defense Systems 97%
- Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence 96%
- Construction of a complete set of Neisseria meningitidis defined mutants - the NeMeSys 2.0 collection - and its use for the phenotypic profiling of the genome of an important human pathogen 95%
Similar papers in this journal
- Comparative phylogenomic analysis reveals evolutionary genomic changes and novel toxin families in endophytic Liberibacter pathogens 95%
- Lysis cassette-mediated exoprotein release in Yersinia entomophaga is controlled by a PhoB-like regulator 93%
- Characterization of TelE, an LXG effector exhibiting a conserved C-terminal glycine zipper motif required for toxicity 93%
Similar papers in this journal
- Aminoglycoside antibiotics inhibit phage infection by blocking an early step of the phage infection cycle 96%
- AcrIF11 is a potent CRISPR-specific ADP-ribosyltransferase encoded by phage and plasmid 95%
- A new role for lipoproteins LpqZ and FecB in orchestrating mycobacterial cell envelope biogenesis 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.