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A reference genome and its epigenetic landscape of potential Orychophragmus violaceus, an industrial crop species

Jia, C.; Hou, Y.; Lai, Q.; Zhang, Y.; Wang, R.; Liu, J.; Wang, J.

2023-09-23 genomics
10.1101/2023.09.21.558835 bioRxiv
Show abstract

Orychophragmus violaceus, also called er-yue-lan in China, is an annual plant of the family of Brassicaceae. The seed oil of O. violaceus contained two specific di-hydroxy fatty acids which were produced by functional divergence between two FAD2 WGD copies determine its industrial property. Here, we assembled a high-quality chromosome-level genome of O. violaceus via based on PacBio CLR technology and its cis-regulatory landscape of five mature tissues, including root, leaf, flower, seed and stem, based on ATAC-seq technology. 1.2 Gb draft genomic sequences were anchored to 12 pseudo-chromosomes and 49904 protein-coding genes were annotated on these chromosomes. To fully understand the epigenetic landscape of O. violaceus, we further performed WGBS-seq for leaf, flower and silique tissues. In total, our multi-omics data provide opportunity to find out the differences between two WGD copies and also a valuable resource for downstream breeding effort of this potential industrial species.

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