Unraveling the role of Xist in X chromosome inactivation: insights from rabbit model and deletion analysis of exons and repeat A
Liang, M.; Zhang, L.; Lai, L.; Li, Z.
Show abstract
X chromosome inactivation (XCI) is a dosage compensation process that aims to equalize the expression of X-linked genes between males and females. Initiation of XCI relies on Xist, which is continuously expressed in somatic cells during XCI maintenance. How Xist impacts XCI maintenance remains unclear, and its functional motifs remain unclear. In this study, we conducted a comprehensive analysis of the Xist. The results demonstrate that rabbits serve as an ideal non-primate animal model for investigating the functions of Xist in vivo. And homozygous knockout of exon 1, exon 6 and repeat A in females resulted in embryonic lethality. However, X{Delta}ReAX females with Xist expression from the intact X chromosome, did not display any abnormalities. Interestingly, there were no significant differences between females with homozygous knockout of exons 2-5 and wild-type rabbits. This suggests that exons 2, 3, 4, and 5 of Xist are less important for XCI. These insights provide valuable knowledge about the functional mechanism of Xist.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Lin28a/let-7 Pathway Modulates the Hox Code via Polycomb Regulation during Axial Patterning in Vertebrates 95%
- EPHA4 signaling dysregulation links abnormal locomotion and the development of idiopathic scoliosis 94%
- Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice. 94%
Similar papers in this journal
- X upregulation is not global and extent of upregulation differs between ancestral and acquired X-linked genes 95%
- Elements of divergence in germline determination in closely related species 93%
- Cell signaling coordinates global Polycomb Repressive Complex 2 recruitment and gene expression in murine embryonic stem cells 93%
Similar papers in this journal
- Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted PGCs 93%
- Developmental regulation of neuronal gene expression by Elongator complex protein 1 dosage. 92%
- Epigenetic regulation of cilia stability and kidney by the chromatin remodeling SWI/SNF complexes 92%
Similar papers in this journal
- Metabolic control of histone acetylation for precise and timely regulation of minor ZGA in early mammalian embryos 94%
- The landscape of mouse epididymal cells defined by the single-cell RNA-Seq 92%
- Light-Sheet Fluorescence Imaging Charts the Gastrula Origin of Vascular Endothelial Cells in Early Zebrafish Embryos 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.