Muscle cell atrophy induced by miR-155-5p reveals molecular targets in skeletal muscle disorders
Lopes, L. O.; Cury, S. S.; de Moraes, D.; Oliveira, J. S.; de Oliveira, G.; Cabral-Marques, O.; Fernandez, G. J.; Hirata, M. H.; WANG, D.-Z.; Dal-Pai-Silva, M.; Carvalho, R.; Freire, P. P.
Show abstract
MicroRNAs are small regulatory molecules that control gene expression. An emerging property of muscle miRNAs is the cooperative regulation of transcriptional and epitranscriptional events controlling muscle phenotype. miR-155 has been related to muscular dystrophy and muscle cell atrophy. However, the function of miR-155 and its molecular targets in muscular dystrophies remain poorly understood. Through in silico and in vitro approaches we identify distinct transcriptional profile of muscle cell atrophy induced by miR-155-5p. The atrophic myotubes changed the expression of 359 genes (166 up-regulated and 193 down-regulated). We reanalyzed muscle transcriptomic data from dystrophin-deficient patients and detected overlap with gene expression patterns in miR-155-treated myotubes. Our analysis indicated that miR-155 regulates a set of transcripts, including Aldh1l, Nek2, Bub1b, Ramp3, Slc16a4, Plce1, Dync1i1, and Nr1h3. Enrichment analysis demonstrates 20 targets involved in metabolism, cell cycle regulation, muscle cell maintenance, and immune system. Moreover, digital cytometry confirmed a significant increase in M2 macrophages, indicating miR-155 effects on immune response in dystrophic muscles. We highlight a critical miR-155 associated with disease-related pathways in skeletal muscle disorders.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Late-Stage Skeletal Muscle Transcriptome in Duchenne muscular dystrophy shows a BMP4-Induced Molecular Signature 96%
- Cholesterol metabolism is a potential therapeutic target in Duchenne Muscular Dystrophy 96%
- RhoA/ROCK2 signalling is enhanced by PDGF-AA in fibro-adipogenic progenitor cells in DMD 95%
Similar papers in this journal
- Immortalised murine R349P desmin knock-in myotubes exhibit a reduced proton leak and decreased ADP/ATP translocase levels in purified mitochondria 92%
- Targeting F-actin stress fibers to suppress the dedifferentiated phenotype in chondrocytes 91%
- RAS isoform specific activities are disrupted by disease associated mutations during cell differentiation 90%
Similar papers in this journal
- Myoparr-associated and -independent multiple roles of heterogeneous nuclear ribonucleoprotein K during skeletal muscle cell differentiation 96%
- Lamin-related congenital muscular dystrophy alters mechanical signaling and skeletal muscle growth 94%
- Postnatal protein intake as a determinant of skeletal muscle structure and function in mice - a pilot study 93%
Similar papers in this journal
- Single-Cell Transcriptome Dynamics of the Autotaxin-Lysophosphatidic Acid Axis During Muscle Regeneration Reveal Proliferative Effects in Mesenchymal Fibro-Adipogenic Progenitors 95%
- Syndecan-4-/- mice have smaller muscle fibers, increased Akt/mTOR/S6K1 and Notch/HES-1 pathways, and alterations in extracellular matrix components 94%
- Functional Characterization of the Lin28/let-7 Circuit during Forelimb Regeneration in Ambystoma mexicanum and its Influence on Metabolic Reprogramming 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.