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Cancer immunotherapy responses persist after lymph node resection

Zhou, H.; Baish, J. W.; O'Melia, M. J.; Darragh, L. B.; Specht, E.; Czapla, J.; Lei, P.-j.; Menzel, L.; Rajotte, J. J.; Nikmaneshi, M. R.; Razavi, M. S.; Vander Heiden, M. G.; Ubellacker, J. M.; Munn, L. L.; Boland, G. M.; Cohen, S.; Karam, S. D.; Padera, T. P.

2023-09-22 cancer biology
10.1101/2023.09.19.558262 bioRxiv
Show abstract

Lymphatic transport facilitates the presentation of cancer antigens in tumor-draining lymph nodes (tdLNs), leading to T cell activation and the generation of systemic anti-cancer immune surveillance. Surgical removal of tdLNs to control cancer progression is routine in clinical practice. However, whether removing tdLNs impairs immune checkpoint blockade (ICB) is still controversial. Our analysis demonstrates that melanoma patients remain responsive to PD-1 checkpoint blockade after regional LN dissection. We were able to recapitulate the persistent response to ICB after regional LN resection in murine melanoma and mammary carcinoma models. Mechanistically, soluble antigen is diverted to distant LNs after tdLN dissection. Consistently, robust ICB responses in patients with head and neck cancer after primary tumor and tdLN resection correlated with the presence of reactive LNs in distant sites. These findings indicate that distant LNs sufficiently compensate for the removal of direct tdLNs and sustain the response to ICB.

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