Probing Molecular Diversity and Ultrastructure of Brain Cells with Fluorescent Aptamers
Lu, X.; Wu, Y.; Li, P.; Fang, T.; Schalek, R. L.; Su, Y.; Carter, J. D.; Gupta, S.; Jain, V.; Janjic, N.; Lichtman, J. W.
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Detergent-free immunolabeling has been proven feasible for correlated light and electron microscopy, but its application is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into recent approaches to delineate neural circuits via connectomics.
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