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Long-term three-dimensional high-resolution imaging of live unlabeled small intestinal organoids using low-coherence holotomography

Lee, M. J.; Lee, J.; Ha, J.; Kim, G.; Kim, H.-J.; Lee, S.; Koo, B.-K.; Park, Y.

2023-09-16 developmental biology
10.1101/2023.09.16.558039 bioRxiv
Show abstract

The prevailing challenges in live unlabeled high-resolution imaging of native organoids stem from technical issues like complex sample handling and optical scattering in three-dimensional architectures. In this study, we introduce low-coherence holotomography as an advanced, label-free, quantitative imaging modality, designed to overcome related technical obstacles for long-term live imaging of 3D organoids. We successfully captured high-resolution morphological intricacies and dynamic events within mouse small intestinal organoids at a subcellular resolution. Furthermore, this method provides a unique advantage in differentiating between viable and non-viable organoids, thereby expanding its potential applications in organoid-based research.

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