Droplet based low input proteomic platform for rare cell populations
Waas, M.; Khoo, A.; Tharmapalan, P.; McCloskey, C. W.; Govindarajan, M.; Zhang, B.; Khan, S.; Waterhouse, P.; Khokha, R.; Kislinger, T.
Show abstract
Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS, an accessible low-input platform that generates high-fidelity proteomic profiles of 100 - 2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, discovering and validating CD36 as a marker of progenitor capacity in the basal cell compartment. We anticipate DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.
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