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Organ-specific prioritization and annotation of non-coding regulatory variants in the human genome

Zhao, N.; Dong, S.; Boyle, A. P.

2023-09-08 bioinformatics
10.1101/2023.09.07.556700 bioRxiv
Show abstract

Identifying non-coding regulatory variants in the human genome remains a challenging task in genomics. Recently, we released the second version of our leading regulatory variant database, RegulomeDB. Building upon this comprehensive database, we developed a novel machine-learning architecture, TLand, which utilizes RegulomeDB-derived features to predict regulatory variants at the cell- or organ-specific level. In our holdout benchmarking, TLand consistently outperformed state-of-the-art models, demonstrating its ability to generalize to new cell lines or organs. We trained three types of organ-specific TLand models to overcome the common model bias toward high data availability cell lines or organs. These models accurately prioritize relevant organs for 2 million GWAS SNPs associated with GWAS traits. Moreover, our analysis of top-scoring variants in specific organ models showed a high enrichment of relevant GWAS traits. We expect that TLand and RegulomeDB will further advance our ability to understand human regulatory variants genome-wide.

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