Propagation of human respiratory syncytial virus in cells derived from the black flying fox (Pteropus alecto).
sugrue, r. J.; Tan, J. B.-H.; Tan, T. J. C.
Show abstract
The propagation of human respiratory syncytial virus (hRSV) was evaluated in the Pteropus alecto kidney (PaKi) cell line. At 20 hrs post-infection, immunoblotting of hRSV-infected PaKi cell lysates with anti-G, anti-N, anti-P and anti-M2-1 indicated expression of the respective virus proteins of the correct size. The hRSV-infected PaKi cell were also stained using anti-F, anti-G, anti-N, anti-P and anti-M2-1 and imaged using immunofluorescence microscopy, which confirmed high levels of virus infection, and the presence of numerous virus filaments and virus-induced inclusion bodies. PaKi cell monolayers also supported multiple cycle infection when hRSV was used to infect PaKi cells using a low multiplicity of infection. These data indicate that prior adaptation of hRSV was not required for its propagation in the PaKi cell line, and suggests that PaKi cell line is a suitable cell model system with which to examine virus-host interactions involving RSV infection in fruit bats.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Negeviruses reduce replication of alphaviruses during co-infection 97%
- Type I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections 95%
- Contribution of segment 3 to the acquisition of virulence in contemporary H9N2 avian influenza viruses 95%
Similar papers in this journal
- Genetic insertion of mouse Myxovirus-resistance gene 1 increases innate resistance against both high and low pathogenic avian influenza virus by significantly decreasing replication in chicken DF1 cell line 96%
- Development of an in vitro model for animal species susceptibility to SARS-CoV-2 replication based on expression of ACE2 and TMPRSS2 in avian cells 95%
- Novel Paju Apodemus Paramyxovirus 1 and 2, Harbored by Apodemus agrarius in The Republic of Korea 94%
Similar papers in this journal
- Isolation and characterization of lumpy skin disease virus from cattle in India 97%
- Development of a reverse transcription polymerase chain reaction for the detection of severe fever with thrombocytopenia syndrome virus from suspected infected animals 95%
- Difference in TMPRSS2 usage by Delta and Omicron variants of SARS-CoV-2: Implication for a sudden increase among children 95%
Similar papers in this journal
- The impact of clade B lineage 5 MERS coronaviruses spike mutations from 2015 to 2023 on virus entry and replication competence 94%
- Neuraminidase antigenic drift of influenza A virus H3N2 clade 3c.2a viruses alters virus replication, enzymatic activity and inhibitory antibody binding 94%
- The alphaherpesvirus conserved pUS10 is important for natural infection and its expression is regulated by the conserved Herpesviridae protein kinase (CHPK) 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.