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Non-invasive measurement of intra-tumoral fluid dynamics with localized convolutional function regression

Woodall, R. T.; Esparza, C. C.; Gutova, M.; Wang, M.; Cunningham-Reynolds, J.; Brummer, A. B.; Stine, C.; Brown, C.; Munson, J. M.; Rockne, R. C.

2023-08-29 bioengineering
10.1101/2023.08.28.554919 bioRxiv
Show abstract

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is a routine method to non-invasively quantify perfusion dynamics in tissues. The standard practice for analyzing DCE-MRI data is to fit an ordinary differential equation to each voxel. Recent advances in data science provide an opportunity to move beyond existing methods to obtain more accurate measurements of fluid properties. Here, we developed a localized convolutional function regression that enables simultaneous measurement of interstitial fluid velocity, diffusion, and perfusion in 3D. We validated the method computationally and experimentally, demonstrating accurate measurement of fluid dynamics in situ and in vivo. Applying the method to human MRIs, we observed tissue-specific differences in fluid dynamics, with an increased fluid velocity in breast cancer as compared to brain cancer. Overall, our method represents an improved strategy for studying interstitial flows and interstitial transport in tumors and patients. We expect that it will contribute to the better understanding of cancer progression and therapeutic response. One-Sentence SummaryA physics-informed computational method enables accurate and efficient measurement of fluid dynamics in individual patient tumors and demonstrates differences between tissues.

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