Spatiotemporal development of growth and death zones in expanding bacterial colonies driven by emergent nutrient dynamics
Kannan, H.; Sun, P.; Caglar, T.; Yao, P.; Taylor, B. R.; Sahu, K.; Ge, D.; Mori, M.; Warren, M.; Kleinfeld, D.; Dong, J.; Li, B.; Hwa, T.
Show abstract
Bacterial colony growth on hard agar is commonplace in microbiology; yet, what occurs inside a growing colony is complex even in the simplest cases. Robust colony expansion kinetics featuring a linear radial growth and a saturating vertical growth indicates a common developmental program which is elucidated here for Escherichia coli cells using a combination of modeling and experiments. Radial colony expansion is found to be limited by mechanical factors rather than nutrients as commonly assumed. In contrast, vertical expansion is limited by glucose depletion inside the colony, an effect compounded by reduced growth yield due to anaerobiosis. Carbon starvation in the colony interior results in substantial cell death within 1-2 days, with a distinct death zone that expands with the growing colony. Overall, the development of simple colonies lacking EPS production and differentiation is dictated by an interplay of mechanical constraints and emergent nutrient gradients arising from obligatory metabolic processes.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Cells reshape habitability of temperature by secreting antioxidants to help each other replicate and avoid population extinction 96%
- Mycobacterium tuberculosis grows linearly at the single-cell level with larger variability than model organisms 95%
- Cell cycle dysregulation of globally important SAR11 bacteria resulting from environmental perturbation 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.