Phospho-signaling couples polar asymmetry and proteolysis within a membraneless microdomain in C. crescentus.
Ahmed, Y. M.; Bowman, G. R.
Show abstract
Asymmetric cell division in bacteria is achieved through cell polarization, where regulatory proteins are directed to specific cell poles. Curiously, both poles contain a membraneless microdomain, established by the polar assembly hub PopZ, through most of the cell cycle, yet many PopZ clients are unipolar and transiently localized. We find that PopZs interaction with the response regulator CpdR is controlled by phosphorylation, via the histidine kinase CckA. Phosphorylated CpdR does not interact with PopZ and is not localized to cell poles. At poles where CckA acts as a phosphatase, de-phosphorylated CpdR binds directly with PopZ and subsequently recruits ClpX, substrates, and other members of a protease complex to the cell pole. We also find that co-recruitment of protease components and substrates to polar microdomains enhances their coordinated activity. This study connects phosphosignaling with polar assembly and the activity of a protease that triggers cell cycle progression and cell differentiation.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- PomX, a ParA/MinD ATPase activating protein, is a triple regulator of cell division in Myxococcus xanthus 97%
- Periplasm homeostatic regulation maintains spatial constraints essential for cell envelope processes and cell viability 96%
- Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq 96%
Similar papers in this journal
- Fluoride triggers lysis in Streptococcus mutans by inhibition of Clp protease complex leading to an unabated competence cascade 96%
- DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria 96%
- Motile ghosts of the halophilic archaeon, Haloferax volcanii 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.