A neurogenetic toolkit to decode Anopheles gambiae olfaction
Giraldo, D.; Hammond, A. M.; Wu, J.; Feole, B.; Al-Saloum, N.; McMeniman, C. J.
Show abstract
The African malaria mosquito Anopheles gambiae exhibits a strong innate sensory drive to seek out human scent. To detect human odorants, An. gambiae uses olfactory sensory neurons (OSNs) that can be divided into different classes by unique repertoires of chemoreceptor gene expression. We applied CRISPR-Cas9-mediated T2A-In Frame Fusions and the QF2/QUAS system to gain genetic access to specific OSN subsets in An. gambiae expressing the chemoreceptor genes Ir25a, Ir76b, Gr22 and orco. We first optimized methods to generate cell-type specific QF2 driver and QUAS responder lines to map expression patterns of these chemoreceptors across mosquito sensory appendages. We next applied transcuticular calcium imaging to record neurophysiological responses to select human-related odorants for each OSN class. This neurogenetic toolkit tiling OSN subsets in An. gambiae, including those responsive to CO2, stands to support systematic efforts to decode olfaction in this prolific disease vector at high-resolution to combat malaria.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species 95%
- Loss of pseudouridine synthases in the RluA family causes hypersensitive nociception in Drosophila 94%
- The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons 94%
Similar papers in this journal
- Marker-assisted mapping enables effective forward genetic analysis in the arboviral vector Aedes aegypti, a species with vast recombination deserts 96%
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae. 96%
- Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editing 95%
Similar papers in this journal
- Evolution of olfactory receptors tuned to mustard oils in herbivorous Drosophilidae 94%
- Unravelling the genetic basis for the rapid diversification of male genital structures between Drosophila species 94%
- Behavioral and genomic sensory adaptations underlying the pest activity of Drosophila suzukii 93%
Similar papers in this journal
- Gene Editing Reveals Obligate and Modulatory Components of the CO2 Receptor Complex in the Malaria Vector Mosquito, Anopheles coluzzii 96%
- Mutagenesis of the Ammonium Transporter AcAmt Reveals a Reproductive Role and a Novel Ammonia-Sensing Mechanism in the Malaria Vector Mosquito Anopheles coluzzii 95%
- Mutagenesis of the Orco Odorant Receptor Co-receptor Impairs Olfactory Function in the Malaria Vector Anopheles coluzzii 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.