The Pvc15 D2-Pnf SP Interaction Mediates AAA ATPase Activity, Payload Stability, and Translocation into the PVC Tube Lumen.
Waterfield, N. R.; Evans, R.
Show abstract
The Photorhabdus virulence cassette (PVC) is an elegant, multi-protein, contractile nanostructure which injects functionally bioactive polypeptides into the eukaryotic cytosol. Signal peptides (SPs) are N-terminal amino acid motifs from native payload proteins which can also associate wide range of heterologous proteins to the PVC tube lumen. In addition, Pvc15 is a classic AAA ATPase encoded within the PVC operon which encodes an N-domain and tandem AAA domains D1 and D2. This work finds that Pvc15s ATPase activity is mediated by the E555 residue situated in the Walker B motif of the sole functional ATPase domain, D2. Pvc15 multimerisation may be required to translocate payload into the PVC tube lumen, which is facilitated by intact N and D1 domains. In addition, ATPase activity requires the presence of other PVC operon components which hints at a PVC-regulated mode of action for loading. Furthermore, D2 is a chaperone for the native Pnf SP, though C-terminal truncations of the Pnf SP confers Pvc15-independent stability to the payload. These findings provide insight into the nuanced roles of the Pvc15-SP interaction for payload-PVC association and the effects that SP length and type has on payload stability and PVC-loading ability.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- An amino-terminal threonine/serine motif is necessary for activity of the Crp/Fnr homolog, MrpC, and for Myxococcus xanthus developmental robustness 95%
- Roles of RodZ and Class A PBP1b in the Assembly and Regulation of the Peripheral Peptidoglycan Elongasome in Ovoid-Shaped Cells of Streptococcus pneumoniae D39 94%
- Transcriptional regulation of the Nε-fructoselysine metabolism in Escherichia coli by global and substrate-specific cues 94%
Similar papers in this journal
- Ser/Thr kinase-dependent phosphorylation of the peptidoglycan hydrolase CwlA controls its export and modulates cell division in Clostridioides difficile 96%
- SmdA is a novel cell morphology determinant in Staphylococcus aureus 95%
- Pyocin S5 import into Pseudomonas aeruginosa reveals a generic mode of bacteriocin transport 95%
Similar papers in this journal
Similar papers in this journal
- Characterization of TelE, an LXG effector exhibiting a conserved C-terminal glycine zipper motif required for toxicity 96%
- A Mycobacterium tuberculosis effector targets mitochondrion, controls energy metabolism and limits cytochrome c exit. 95%
- Lysis cassette-mediated exoprotein release in Yersinia entomophaga is controlled by a PhoB-like regulator 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.