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Electrically controlled interaction between cable bacteria and carbon electrodes

Bonne, R.; Marshall, I. P. G.; Bjerg, J.; Marzocchi, U.; Manca, J.; Nielsen, L. P.; Aiyer, K.

2023-08-14 microbiology
10.1101/2023.08.14.553267 bioRxiv
Show abstract

Cable bacteria couple the oxidation of sulphide in sediments to the reduction of oxygen via long-distance electron transfer through periplasmic wires. While direct electron transfer between cable bacteria cells belonging to the same filament is a well-known phenomenon, electron transfer from the filament to electrodes has remained elusive. In this study, we demonstrate that living cable bacteria are attracted to electrodes in different bioelectrochemical systems. Carbon felt and carbon fibre electrodes poised at +200 mV against an Ag/AgCl reference attracted live cable bacteria from the sediment. When the applied potential was switched off, cable bacteria retracted from the electrode. qPCR and scanning electron microscopy corroborated this finding and revealed cable bacteria adhered onto the electrode surface. These experiments raise new possibilities to cultivate cable bacteria and utilise them for important applications in bioelectrochemical systems.

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