DHX36 binding induces RNA structurome remodeling and regulates RNA abundance via m6A/YTHDF1
Zhang, Y.; Zhao, J.; Chen, X.; Lyu, K.; Ding, Y.; Sun, H.; Kwok, C. K.; Wang, H.
Show abstract
RNA structure constitutes a new layer of gene regulatory mechanisms. RNA binding proteins can modulate RNA secondary structures, thus participating in post-transcriptional regulation. The DEAH-box helicase 36 (DHX36) is known to bind and unwind RNA G-quadruplex (rG4) structure but the transcriptome-wide RNA structure remodeling induced by DHX36 binding and the impact on RNA fate remain poorly understood. Here, we investigate the RNA structurome alteration induced by DHX36 depletion. Our findings reveal that DHX36 binding induces structure remodeling not only at the localized binding sites but also on the entire mRNA transcript most pronounced in3UTR regions. DHX36 binding increase structural accessibility at 3UTRs which is correlated with decreased post-transcriptional mRNA abundance. Further analyses and experiments uncover that DHX36 binding sites are enriched for N6-methyladenosine (m6A) modification and YTHDF1 binding; and DHX36 induced structural change may facilitate YTHDF1 binding to m6A sites leading to RNA degradation. Altogether, our findings uncover the structural remodeling effect of DHX36 binding and its impact on RNA abundance through regulating m6A dependent YTHDF1 binding.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase 96%
- RNA binding protein PRRC2B mediates translation of specific mRNAs and regulates cell cycle progression 95%
- Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6 95%
Similar papers in this journal
- In vivo structure and dynamics of the RNA genome of SARS-Cov-2 95%
- The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesis 95%
- SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1-XBP1 Signaling Under ER Stress 95%
Similar papers in this journal
Similar papers in this journal
- Proteins with amino acid repeats constitute rapidly evolvable and human-specific essentialome 94%
- Histone H1 regulates non-coding RNA turnover on chromatin in a m6A-dependent manner 94%
- LARP1 facilitates translational recovery after amino acid refeeding by preserving long poly(A)-tailed TOP mRNAs 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.