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Design, Development, And Validation Of A Nanobiosensor To Detect Circulating Microbiome For Cardiovascular Disease Risk Assessment

Nazeer, N.; Dewangan, R.; Zaidi, K.; Gurjar, V.; Tiwari, R.; Mishra, P. K.

2023-08-08 cardiovascular medicine
10.1101/2023.08.05.23293588 medRxiv
Show abstract

Cardiovascular disease (CVD) is a serious worldwide health concern that necessitates the development of novel diagnostic techniques for early identification and personalized healthcare management. Even before the insights provided by gut microbiota, current research has demonstrated the importance of circulating microbiome (CMB) in the evolution of cardiometabolic illness risk and progression. We developed a nanobiosensor that uses specific labeled capture probes with perovskite quantum dots (PQDs) to detect the targeted 16S rRNA sequences in the peripheral milieu. With ideal applicability, specificity, and sensitivity, this sensor delivers unique insights into the presence and characterization of circulating microbiota signatures. Developing a nanophotonic microbiome detection method in body fluids may pave the way for creating a distinctive tool for CVD risk prediction for population-based screening programs in low and middle-income countries.

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