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m6A mRNA Methylation Regulates Early Pancreatic β-Cell Differentiation

Kahraman, S.; De Jesus, D. F.; Wei, J.; Brown, N. K.; Zou, Z.; Hu, J.; He, C.; Kulkarni, R. N.

2023-08-03 developmental biology
10.1101/2023.08.03.551675 bioRxiv
Show abstract

N6-methyladenosine (m6A) is the most abundant chemical modification in mRNA, and plays important roles in human and mouse embryonic stem cell pluripotency, maintenance, and differentiation. We have recently reported, for the first time, the role of m6A in the postnatal control of {beta}-cell function in physiological states and in Type 1 and 2 Diabetes. However, the precise mechanisms by which m6A acts to regulate the development of human and mouse {beta}-cells are unexplored. Here, we show that the m6A landscape is dynamic during human pancreas development, and that METTL14, one of the m6A writer complex proteins, is essential for the early differentiation of both human and mouse {beta}-cells.

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