Optogenetic control of lysosome function
Ilyinsky, N. S.; Bukhalovich, S. M.; Bagaeva, D. F.; Nesterov, S. V.; Alekseev, A. A.; Tsybrov, F. M.; Bogorodskiy, A. O.; Alekhin, V. A.; Nazarova, S. F.; Moiseeva, O. V.; Vlasova, A. D.; Kovalev, K. V.; Mikhailov, A. E.; Rogachev, A. V.; Bamberg, E.; Borshchevskiy, V. I.; Gordeliy, V. I.
Show abstract
Lysosome protective, metabolic, signaling functions are highly dependent on their pH. A lack of tools of high spatial and temporal resolution for pH control is a bottleneck of lysosome related cell research. Light-driven inward proton pump NsXeR, targeted to the lysosomes of mammalian cells, produces lysosome alkalization simply by light. Complementary use of outward proton pumping Arch3 rhodopsins in lysosomes offers an approach to vary pH in a range from around 5 to 6.5 in both directions (de-acidification and acidification). Lyso-NsXeR optogenetics efficiency was demonstrated, in particular, by its ability to inhibit lysosome proteolytic enzymes. Thus, optogenetic monitoring and regulation of the lysosome function, through pH control over a wide range, could serve as an approach to studying fundamental cell processes, and rational drug design.
Matching journals
The top 11 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Reversing protonation of weakly basic drugs greatly enhances intracellular diffusion and decreases lysosomal sequestration 93%
- Opto-RhoGEFs: an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength 92%
- A green lifetime biosensor for calcium that remains bright over its full dynamic range 92%
Similar papers in this journal
- VISION -- an open-source software for automated multi-dimensional image analysis of cellular biophysics 93%
- Light Activated BioID (LAB): an optically activated proximity labeling system to study protein-protein interactions. 92%
- ExoJ: an ImageJ2/Fiji plugin for automated spatiotemporal detection and analysis of exocytosis 91%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.