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Selective elimination of CD169+ macrophages in lymph nodes invaded by breast cancers

Maeshima, Y.; Kataoka, T. R.; Vandenbon, A.; Hirata, M.; Takeuchi, Y.; Suzuki, Y.; Fukui, Y.; Ibi, Y.; Haga, H.; Morita, S.; Toi, M.; Kawaoka, S.; Kawaguchi, K.

2023-08-05 cancer biology
10.1101/2023.08.02.551659 bioRxiv
Show abstract

Breast cancer cells suppress the host immune system to efficiently invade the lymph nodes; however, the underlying mechanism remains incompletely understood. Here, we report that metastasized breast cancer cells selectively eliminate CD169+ lymph node sinus macrophages, an initiator of anti-cancer immunity, from the lymph nodes. The comparison between paired lymph nodes with and without metastasis from the same patients demonstrated that the number of CD169+ macrophages was reduced in metastatic lymph nodes, whereas the numbers of other major immune cell types were unaltered. We also detected the infiltration of CD169+ macrophages into metastasized cancer tissues depending on sections, suggesting that CD169+ macrophages were gradually eliminated after anti-cancer reactions. Furthermore, CD169+ macrophage elimination was prevalent in major breast cancer subtypes and correlated with breast cancer staging. Collectively, we propose that metastasized breast cancer cells dispel CD169+ macrophages from lymph nodes in a phased manner, disabling a critical step of anti-cancer immunity.

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