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Time-deterministic cryo-optical microscopy

Yamanaka, M.; Tsuji, K.; Kumamoto, Y.; Tamura, S.; Miyamura, W.; Kubo, T.; Mizushima, K.; Kono, K.; Hirano, H.; Sugiura, K.; Fukushima, S.-i.; Kunimoto, T.; Nishida, K.; Mochizuki, K.; Harada, Y.; Smith, N. I.; Heintzmann, R.; Nagai, T.; Tanaka, H.; Fujita, K.

2023-08-03 biophysics
10.1101/2023.08.01.551103 bioRxiv
Show abstract

We developed a technique to rapidly freeze biological systems during observation under an optical microscope, which allows for detailed optical observation at a precise time in the biological dynamics at high spatial resolution and quantifiability under low-temperature conditions. Our study further found that ion distributions and molecular conformations can be fixed in space and time, demonstrated by visualization of calcium waves rapid-frozen at an instant using fluorescent ion indicators. These results show that time-deterministic cryo-optical microscopy can suspend cellular dynamics while maintaining molecular and ionic states, allowing us to view cellular dynamics in a way that has not been possible before. One-Sentence SummaryRapid freezing of dynamic biological systems during optical observation presents time information to the fixed samples

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.