Back

Square beams for optimal tiling in TEM

Chua, E. Y. D.; Alink, L. M.; Kopylov, M.; Eisenstein, F.; Johnston, J. D.; de Marco, A.

2023-10-09 biophysics
10.1101/2023.07.29.551095 bioRxiv
Show abstract

Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application, showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups.

Matching journals

The top 5 journals account for 50% of the predicted probability mass.

1
Nature Methods
○ 385 papers in training set
Top 0.5%
15.1%
2
IUCrJ
● 32 papers in training set
Top 0.1%
11.0%
3
eLife
● 5828 papers in training set
Top 10%
9.8%
4
Journal of Structural Biology
○ 64 papers in training set
Top 0.1%
9.7%
5
Nature Communications
○ 5641 papers in training set
Top 19%
8.9%
50% of probability mass above
6
Microscopy and Microanalysis
◐ 12 papers in training set
Top 0.1%
7.3%
7
Cell Reports Methods
○ 165 papers in training set
Top 1%
2.4%
8
Journal of Microscopy
○ 20 papers in training set
Top 0.1%
2.1%
9
Proceedings of the National Academy of Sciences
● 2444 papers in training set
Top 27%
1.7%
10
Optica
● 27 papers in training set
Top 0.2%
1.7%
11
Journal of Applied Crystallography
● 14 papers in training set
Top 0.1%
1.7%
12
Journal of Structural Biology: X
○ 17 papers in training set
Top 0.1%
1.5%
13
Science
● 477 papers in training set
Top 6%
1.5%
14
Communications Biology
○ 993 papers in training set
Top 18%
1.4%
15
Development
● 497 papers in training set
Top 4%
1.3%
16
Bioinformatics
◐ 1204 papers in training set
Top 8%
1.1%
17
Nature Biotechnology
○ 172 papers in training set
Top 3%
1.1%
18
Scientific Reports
○ 3612 papers in training set
Top 65%
1.1%
19
Nano Letters
● 71 papers in training set
Top 1.0%
1.1%
20
Small Methods
○ 29 papers in training set
Top 0.4%
1.1%
21
Nature Photonics
○ 10 papers in training set
Top 0.1%
1.0%
22
Acta Crystallographica Section D Structural Biology
● 59 papers in training set
Top 0.4%
1.0%
23
Wellcome Open Research
○ 67 papers in training set
Top 2%
0.8%
24
Structure
○ 193 papers in training set
Top 2%
0.8%
25
Cell Systems
○ 201 papers in training set
Top 5%
0.8%
26
Biophysical Reports
○ 37 papers in training set
Top 0.6%
0.6%
27
Light: Science & Applications
○ 16 papers in training set
Top 0.3%
0.6%
28
ACS Photonics
● 13 papers in training set
Top 0.2%
0.6%
29
Science Advances
● 1243 papers in training set
Top 33%
0.6%