Correlative superresolution microscopy with deep UV reactivation
Prakash, K.
Show abstract
Correlative superresolution microscopy has the potential to accurately visualize and validate new biological structures past the diffraction limit. However, combining different superresolution modalities, such as deterministic stimulated emission depletion (STED) and stochastic single-molecule localization microscopy (SMLM), is a challenging endeavor. For correlative STED and SMLM, the following poses a significant challenge: (1) the photobleaching of the fluorophores in STED; (2) the subsequent reactivation of the fluorophores for SMLM; and (3) finding the right fluorochrome and imaging buffer for both imaging modalities. Here, we highlight how the deep ultraviolet (DBUE) wavelengths of the Mercury (Hg) arc lamp can help recover STED bleaching and allow for the reactivation of single molecules for SMLM imaging. We also show that Alexa Fluor 594 and the commercially available Prolong Diamond turn out to be excellent fluorophores and imaging media for correlative STED and SMLM.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation 96%
- An evaluation of multi-excitation-wavelength standing-wave fluorescence microscopy (TartanSW) to improve sampling density in studies of the cell membrane and cytoskeleton 95%
- Ultra-thin fluorocarbon foils optimize multiscale imaging of three-dimensional native and optically cleared specimens 95%
Similar papers in this journal
- Fluorescence fluctuation based super resolution microscopy, basic concepts for an easy start. 96%
- Robust optical autofocus system utilizing neural networks trained for extended range and time-course and automated multiwell plate imaging including single molecule localization microscopy 95%
- A comparison of super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium 95%
Similar papers in this journal
- A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics. 94%
- Correlative all-optical quantification of mass density and mechanics of sub-cellular compartments with fluorescence specificity 94%
- Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.