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Dearth of smoking-induced mutations in oncogene-driven non-small-cell lung cancer despite smoking exposure

Huang, C.-Y.; Jiang, N.; Shen, M.; Lai, G.; Tan, A. C.; Jain, A.; Saw, S. P.; Ang, M.-K.; Ng, Q. S.; Lim, D. W.-T.; Kanesvaran, R.; Tan, E.-H.; Tan, W. L.; Ong, B.-H.; Chua, K. L.; Anantham, D.; Takano, A.; Lim, T. K. H.; Tam, W. L.; Sim, N. L.; Sanderup, A. J.; Tan, D. S. W.; Rozen, S. G.

2023-07-06 cancer biology
10.1101/2023.07.04.547310 bioRxiv
Show abstract

Non-small cell lung cancers (NSCLCs) in non-smokers are mostly driven by mutations in the oncogenes EGFR, ERBB2, and MET, and fusions involving ALK and RET. We term these "non-smoking-related oncogenes" (NSROs). In addition to occurring in non-smokers, NSRO-driven tumors also occur in smokers, and the clonal architecture and genomic landscape of these tumors remain unknown. We investigated genomic and transcriptomic alterations in 173 tumor sectors from 48 patients with NSRO-driven or typical-smoking NSCLCs. NSRO-driven NSCLCs in smokers and non-smokers have similar genomic landscapes. Surprisingly, even in patients with prominent smoking histories, the mutational signature caused by tobacco smoking was essentially absent in NSRO-driven NSCLCs. However, NSRO-driven NSCLCs in smokers had higher transcriptomic activities related to regulation of the cell cycle, suggesting that smoking still affects tumor phenotype independently of genomic alterations. Statement of significanceThis study highlights the lack of genomic scars caused by smoking in NSCLCs driven by non-smoking-related oncogenes regardless of smoking history. The impact of smoking on these tumors is mainly non-genomic. The transcriptomic features of NSCLCs associated with smoking may help in the development of therapeutic approaches.

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